Phytoremediation using an indigenous crop plant (wheat): The uptake of methyl parathion and metabolism of P-nitrophenol

Khan, Nayeem Ullah and Varma, Bhavya and Imrana, Nazeeb and Shetty, Paddu Krishnappa (2011) Phytoremediation using an indigenous crop plant (wheat): The uptake of methyl parathion and metabolism of P-nitrophenol. Indian Journal of Science and Technology, 4 (12). pp. 1-7. ISSN 0974- 6846

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    Abstract: Phytoremediation is an emerging technology for degradation of organophosphate pesticides. Rhizofiltration, soil microorganisms and rhizosphere play an important role in phytoremediation. For this purpose, experiments were carried out to analyze the pesticide uptake by plants and residue in the soil under controlled and experimental conditions. Organophosphate pesticide binds to both organic matter and antibiotic streptomycin. Streptomycin inhibits the activity of the enzyme (p-nitrophenol 4-hydroxylase) extracted from root and shoot. The results obtained from UVvisible spectrophotometer were confirmed by HPLC analysis. Wheat plants enhanced uptake/degradation of methyl parathion, p-nitrophenol and hydroquinone in unsterilized soil by 64.85%, 94.7% and 55.8% respectively. Methyl parathion hydrolyzes to p-nitrophenol, which is further metabolized to hydroquinone with nitrite release. The enzyme pnitrophenol 4-hydroxylase is active as evidenced by release of nitrite by leaf and root extracts and also by the appearance of hydroquinone in the reaction mixture.
    Item Type: Article
    Additional Information: Copyright belongs to the Publisher
    Keywords: Phytoremediation, Streptomycin, methyl parathion, p-nitrophenol, hydroquinone, pesticide degradation
    Subjects: School of Natural and Engineering Sciences > Environment
    Divisions: Schools > Natural Sciences and Engineering
    Date Deposited: 29 Nov 2011 07:25
    Last Modified: 08 May 2015 09:53

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